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Blood samples
 
For taking of blood samples, use only of Ketaminhydrochloride is recommended because additional tranquilizers can influence the result. Stress, caused by capture and restraint without use of tranquilizers, can also influence results 67. Obtaining of blood-samples from lorises and pottos with a syringe is difficult 15, 34, possibly because of the peculiarities of blood vessels of the limbs. Blood samples can be obtained from the jugular or femoral vein; the jugular vein is rarely visible or palpable, localization is based on approximation and pulsations from the carotid artery 61.
 
At Ruhr-University, laboratory-bred blood-suckling bugs (Dipetalogaster maxima) are used for taking blood samples. The lorises are allowed to enter a small wiremesh cage with food and some cover (artificial plants) before the end of the sleeping period and are enclosed in it by a shutter. They try to escape from the cage for a few minutes, then calm down and sit down for sleeping. In a compartment adjacent to the sleeping branch, separated from the loris by wiremesh and very thin cloth against which the loris leans during sleeping, a bug is hidden in a box from which it can be released by pulling a thread after the loris has settled down, then comes out and suckles blood through the cloth hiding it. By chosing subadult bugs of a certain size, the amount of blood can be predetermined. The lorises, even when still awake, do not notice that blood is taken from them. When the bug is stout with blood, the loris is released through the shutter, gets a titbit, and the bug can be caught for obtaining the blood which is unchanged with exception of a slight aggregation of thrombocytes (Schaub, pers. comm). Blood obtained in this way can also be used for taxonomic purposes (Yves Rumpler, pers. comm.). Detection of a host by the bugs partly depends on a temperature difference between host and environment; when ambient temperature was too high, the bugs were not successful. A publication about this method for taking blood samples is in preparation by Prof. Schaub, Ruhr-Universität Bochum, he also provided the bugs for our study.

 
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